DNA Sequencing
Next Generation of DNA Sequencing.
Overview of DNA Sequencing for automated sequencers.
DNA cycle sequencing.
Animation (to view, click play for the cycle sequencing animation.)
SOLiD System - Next-Generation Sequencing!
Sequencing primers
Many sequencing primers are commercially available for T7, Sp6, T3, M13 Forward and M13 Reverse recognition sites.
Chemical synthesis of DNA primer.
Analyzing DNA sequencing results
http://www.ocf.berkeley.edu/~edy/genome/sequencing.html
Look at even spacing.
Compressions usually result from repeated homopolymers of C and Gs that are forming secondary structure.
Can use dITP instead of dGTP to interupt secondary structure formation.
In diploid organisms, single nucleotide polymorphisms (snps) can be observed.
Maxam - Gilbert Direct chemical Sequencing of labeled, single-stranded DNA
Direct chemical sequencing of labeled RNA
Direct enzymatic sequencing of labeled RNA
