Introduction to Recombinant Genetics- Biology 350

Assignment

The p53 gene is a tumor suppressor gene, i.e., when p53 is non-functional, oncogenic expressions increase. What information would you like to know about the p53 gene? List as many as possible.

Here is the 2005 Class responses.

Gene Function

Bioinformatics

-Match gene sequence with those with a known function

-Match segments or domains to subfunctions (e.g., NTP binding site)

Transcription

-Transcript Length => Northern Blot

-Promoter, terminator, or Splicing alternatives => Northern Blot

-Start and stop sites => Northern Blot, Primer extension w/RTase

-Tissue => Northern Blot, RT-PCR

-Temporal Expression => Northern Blot, RT-PCR

-Regulatory Elements => Nuclease protection, Deletion assays, Gel retardation, DNaseI footprinting

Translation

-Size and properties of protein => In vitro translation, Gel Electrophoresis, Centrifugation, Enzyme activity.

-Similar to other proteins => Immunoprecipitation

-Single or multiple subunits =>Western Blot

Protein

-Protein Properties =>

Function

Is a function tied to this protein => Deletion Analysis