Introduction to Recombinant Genetics- Biology 350

X-ray crystallography

2d and 3d nmr

Auremol, Automated structure determination from NMR Data in Solution.

Electron microscopy

Cryoelectron microscopy

Maldi and rapid identification

Protein identification usually requires that the protein be separated from all other protein before identification can be done.

Question:
What method(s) would you use to separate proteins from a tissue into its pure peptide components?

Mass Analysis

Matrix-assisted Laser Desorption/Ionisation (MALDI)