DNA Replication

(Lodish et al., 2000, Section  12.1, 12.2)

DNA replication.

DNA replication is semiconservative (Fig. 12.1).
DNA replication begins at specific chromosomal sites called origins (Fig. 12.5).
    Prokaryotes have a single origin.
    Eukaryotes have many origins.
Initiation of DNA replication begins as proteins recognize and bind origins forming an open complex.
    E. coli origin recognition (Fig. 12.7).  = Prokaryote example.
    SV40 origin recognition (Fig. 12.12).  = Eukaryote example.
Growing replication fork (Fig. 12.9a).
    Bond formation (movie).
    Leading and Lagging Strand Formation (movie).
    E. coli  (movie).
        Components of replication complex (Fig 12.11).
        Lagging-strand synthesis (Fig. 12.9b).
        Ligase reaction (Fig. 12.9c).
    SV40  (Fig. 12.12).  = Eukaryote example.
Replicating the ends of linear DNA (Fig. 12.13) (movie).
Properties of DNA Polymerases (Table 12.1).

Activity

Quiz


References:
Lodish, H., Berk, A., Zipursky, S.L., Matsudaira, P., Baltimore, D., Darnell, J., 2000, Molecular Cell Biology, 4th Ed., W.H. Freeman and Company, NY, New York.  ISBN 0-7167-3136-3.