Immunology / Sample Exam #4 Spring
2001
Cytokine Matching
| a) IL-1 | g) IL-10 |
| b) IL-2 | h) TNFa |
| c) IL-3 | i) TNFb |
| d) IL-4 | j) IL-12 |
| e) IL-5 | k) IL-18 |
| f) IL-6 |
1. Originially known as “T cell growth factor”, this Th1 cytokine stimulates the growth and proliferation of Th1 cells.
2. Originially known as “cytokine synthesis inhibitory factor”, this Th2 cytokine down regulates macrophage cytokine synthesis which, in turn, functions to down regulate Th1 cytokine synthesis.
3. This cytokine is secreted by both Th1 and Th2 cells and is believed to function at the very early stages of hematopoiesis.
4. This Th2 cytokine promotes the heavy class switch to IgA and promotes the growth and differentiation of eosinophils.
5. This Th2 cytokine promotes the activation, proliferation, and differentiation of B lymphocytes and is responsible for triggering the Heavy class switch from IgM---->IgG1 and from IgG1-----> IgE.
6. When present at elevated levels, this cytokine causes cachexia and is responsible for the physiological symptoms associated with Gram - septic shock.
7. This pro-inflammatory cytokine, which exists in both membrane-bound and secreted form, also functions as an endogenous pyrogen and as a co-stimulatory signal for Th cell proliferation following antigen presentation.
8. This APC cytokine is believed to be responsible for driving the differentiation of an uncommitted Th0 lymphocyte to the Th1 subset.
Multiple Choice Questions (1 pt each)
9. Greater than 95% of T lymphocytes found in the blood and lymph have which form of the TcR?
a) a b
b) g d
c) g e
d) a g
10. Which of the following investigators first succesfully cloned the genes for the TcR using the technique of subtractive hybridization?
a) Hood and Leder
b) Tongegawa Hozumi
c) Dryer and Bennett
d) Hedrick and Davis
11. Which of the following is NOT a Th2 cytokine?
a) IL-2
b) IL-4
c) IL-5
d) IL-10
12. Which of the following is NOT one of the four structural families of cytokines:
a) hematopoietin family
b) chemokine family
c) interleukin family
d) tumor necrosis factor family
13. CD4 and CD8 both:
a) bind to Class II MHC
b) function in signal transduction
c) bind to B7
d) a & b
14. The ITAM sequence is found within the:
a) transmembrane domains of
the a b chains of the TcR
b) cytoplasmic domains of the a b TcR and g d TcR
c) cytoplasmic domains of CD4 and CD8
d) cytoplasmic domains of all 5 CD3 glycoproteins
15. Which of the following does NOT contribute to the diversity of the the TcRs?
a) somatic mutation
b) V-J, V-D-J rearrangement
c) N- nucleotide addition
d) Junctional Flexibility
16. Multiple V, D, and J gene segments are found within the germline configuration of:
a) a and b chains
b) b and d chains
c) g and d chains
d) b and g chains
17. The TcR and immunoglobulins share many of the same mechanism for generating diversity in the antigen binding site. Which of the following mechanisms contribute to the diversity of the TcR but do not contribute to the diversity of Ig?
a) somatic mutation
b) V-J and V-D-J rearrangement
c) junctional flexibility
d) D region diversity
18. Which of the following pairs of cytokines could be said to have antagonistic activities?
a) IL-1 and TNF-a
b) IL-12 and IFN-g
c) IL-10 and IFN-g
d) IL-4 and IL-5
19. Which of the following pairs of cytokines could be said to have redundant activities?
a) IL-1 and TNF-a
b) IL-10 and IFN-g
c) IL-1 and IL-3
d) TNF-a and IL-2
20. Which of the following cytokines does NOT function as an endogenous pyrogen?
a) IL-2
b) IL-1
c) IL-6
d) TNF-a
21. The antigen binding site of the ab TcR is formed by:
a) the b1 and b2 domains
b) the Va and Vb domains
c) the Va and Ca domains
d) the a1 and b1 domains
22. The CD4 glycoprotein is found:
a) on all Tc lymphocytes
b) only on Th1 lymphocytes
c) on all Th lymphocytes
d) on all NK cells
23. A T lymphocyte which expresses CD3 must also express:
a) the TcR
b) CD4
c) Class II MHC
d) membrane Ig
24. The TcR is found on:
a) all mature lymphocytes
b) all mature T lymphocytes
} c) all mature B lymphocytes
d) all NK cells
25. Which of the following comprise the high affinity receptor for IL-2?
a) gd
b) ag
c) abg
d) bg
26. CD40 on the surface of B lymphocytes binds to which of the following Th cell surface molecules?
a) ICAM
b) TcR
c) CD40L
d) CD16
27. Which of the following components of complement possess the binding site for the Fc portion of IgG or IgM?
a) C1s
b) C9
c) C1q
d) C3
28. C1 is composed of all of the following except:
a) C1q
b) C1r
c) C1s
d) C1b
29. Which of the following complement components assumes a "bouquet of tulips" conformation?
a) C9
b) C3b
c) C1q
d) C1s
30. C1r serves as a catalyst for which of the following reactions?
a) C1s-------->C1s
b) C3--------->C3b + C3a
c) C2--------->C2a + C2b
d) C4--------->C4b + C4a
31. Which of the following is considered to be a C5 convertase?
a) C4bC2a
b) C1s
c) C4bC2aC3b
d) C3bB
32. Which of the following reactions is an important amplification step in the complement cascade?
a) C1s--------->C1s
b) C4----------->C4b + C4a
c) C3---------->C3b + C3a
d) C2---------->C2a + C2b
33. Which of the following complement components is not common to both the alternative and classical pathways:
a) C3b
b) C5b
c) C9
d) C4b
34. C3bBbC3b has a similar function as which of the following from the classical pathway?
a) C4bC2a
b) C4bC2aC3b
c) C1r
d) C5bC6C7
35. Which of the following components initiates formation of the membrane attack complex when bound to the target cell surface?
a) C2b
b) C3b
c) C4b
d) C5b
36. Which of the following complement components is actually a perforin-like molecule?
a) C5b
b) C9
c) C8
d) C7
37. Which of the following regulatory proteins functions at the latest stage in the complement cascade?
a) Factor H
b) Factor I
c) C4b binding protein
d) S protein
38. Decay accelerating factor acts on which of the following steps of the cascade:
a) C1s-------->C1s
b) C4---------->C4b + C4a
c) C4bC2a
d) C5bC6C7
39. Which of the following regulatory proteins can prevent C3b from binding to Factor B?
a) S protein
b) decay accelerating factor
c) Factor H
d) C4b binding protein
40. All of the following
function as anaphylatoxins except:
a) C2a
b) C3a
c) C4a
d) C5a
41. Which of the following complement components attracts neutrophils to the sites of complement activity ?
a) C3b
b) C2a
c) C5a
d) C4a
42. CTL cells are usually:
a) CD4+
b) CD8+
c) CD4-CD8-
d) CD4+CD8+
43. Which of the following is not true of CTL-Ps?
a) they lack IL-2 receptors
s
b) they lack the ability to proliferate
c) they are incapable of lysing target cell
d) they lack TcRs
44. Which of the following cytokines has been shown to be most important in the regulation of CTL activity?
a) IL-1
b) IL-2
c) IL-4
d) IFN-g
45. Which of the following molecules are not typically found within CTL granules:
a) TNFbs
b) granzymes
c) histamines
d) perforins
46. CD16 appears to be an important cell surface marker for:
a) eosinophils
b) NK cells
c) neutrophils
d) macrophages
47. The current hypothesis is that NK cells recognize target cells:
a) because of their TcR specificity
b) due to low levels of Class I MHC expression on target cells
c) due to the presence of sialic acid on the surface of target cells
d) because the target cells are displaying viral peptides or tumor specific
antigens
48. Which of the following best describes NK cell morphology?
a) small TcR- lymphocytes
b) large granular lymphocytes
c) large polymorphonuclear leukocytes
d) large Ig+ lymphocytes
Short Answer Questions:
A B cell must receive at least
2 types of signals before differentiating into plasma cells or memory
B cells, in the space below give two required signals.
List below 3 characteristics
of the antibody response to T independent antigens.
ADCC is an acronym that represents
what immunological process? (2pts)
List 4 cells that participate in ADCC (2pts)
You are a clinical immunologist who has diagnosed two patients with complement deficiencies. Patient 1 has no functional C3. Patient 2 is lacking functional C1. Which patient would be most negatively impacted? Why? (5pts)