| Introduction to Recombinant Genetics- Biology 350 | |
|
| Laboratory Index Page | ||
| Lecture Index | ||
Date |
Topics |
Book Chapter |
Sept. 1 |
Introduction -information resources, stats | |
3 |
Genetic Organization | |
8 |
What is Recombinant Gene Technology | 1, 2 |
10 |
Restriction enzymes and Ligases | 4 |
15 |
Restriction enzyme maps | 4 |
17 |
DNA Isolation -purpose, types of clones, source of material | 3 |
22 |
Phage isolation | 3 |
24 |
Enzyme tools needed for cloning | 4, + Handout |
29 |
Cloning vectors for E.coli & other bacteria | 6 |
25 |
Cloning vectors for Eukaryotes | 7 |
Oct. 1 |
Introduction of DNA into cells | 5 |
6 |
Hybridization techniques |
8 |
13 |
DNA sequencing , primer synthesis | 10 |
15 |
Sequence assembly | 12 |
20 |
Determining coding potential of a sequence | 12 |
22 |
Determining the function of an unknown gene | 12 |
27 |
Studying transcription, Northerns, microarrays, RT-PCR | 11 |
29 |
Proteome Analysis& 2 hybrid system | 12 |
Nov. 3 |
Metabolomics Making antibodies against proteins and their use |
|
5 |
Mutagenesis | |
10 |
Knockouts and siRNA inhibition of gene expression | 15 |
12 |
Protein expression ...continued. | 13 |
17 |
Isolation
of purified recombinant proteins Determining protein structure, MALDI |
13 |
19 |
Basics in Forensics |
16 |
24 |
...continued | |
Dec. 1 |
Phylogenetics and Species identification | 16 |
3 |
Gene Therapy | 14 |
8 |
Project | |
10 |
Project | |
Dec. 17 |
Final Exam 10:30 - 12:30 AM | |
| © 2005 by CA Rinehart | Index • Syllabus • CourseInfo LogIn • References • t |
| This material is intended for use only by WKU students registered for Biology 350. Other uses prohibited. | |