| Introduction to Recombinant Genetics- Biology 350 | |
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| Syllabus Fall 2009, 9:35 - 10:35, Tuesdays and Thursdays 3109 EBS |
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| Instructor: Claire Rinehart Office: 121 TCNW Phone: 270-745-5997 Office Hours: by appointment, emailclaire.rinehart@wku.edu |
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| Textbook: Gene Cloning and DNA analysis, An Introduction by T.A. Brown. |
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Date |
Topics |
Book Chapter |
Sept. 1 |
Introduction -information resources, stats | |
3 |
Genetic Organization | 1, 16 |
8 |
What is Recombinant Gene Technology | 2 |
10 |
Restriction enzymes and Ligases | 3 |
15 |
Restriction enzyme maps | |
17 |
DNA Isolation -purpose, types of clones, source of material | |
22 |
Phage isolation | 4 |
24 |
Enzyme tools needed for cloning | 6, + Handout |
29 |
Cloning vectors for E.coli & other bacteria | 4, 5, 10 |
25 |
Cloning vectors for Eukaryotes | 11, 14 |
Oct. 1 |
Introduction of DNA into cells | 12 |
6 |
Hybridization techniques |
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13 |
DNA sequencing , primer synthesis | 7 |
15 |
Sequence assembly | 17 |
20 |
Determining coding potential of a sequence | |
22 |
Determining the function of an unknown gene | 9 |
27 |
Studying transcription, Northerns, microarrays, RT-PCR | 20 |
29 |
Proteome Analysis& 2 hybrid system | |
Nov. 3 |
Metabolomics Making antibodies against proteins and their use |
24 |
5 |
Mutagenesis | 8, 19 |
10 |
Knockouts and siRNA inhibition of gene expression | 13, 15 |
12 |
Protein expression ...continued. | 21 |
17 |
Isolation
of purified recombinant proteins Determining protein structure, MALDI |
22, 23 |
19 |
Basics in Forensics |
26 |
24 |
...continued | |
Dec. 1 |
Phylogenetics and Species identification | 18 |
3 |
Gene Therapy | |
8 |
Project | |
10 |
Project | |
Dec. 17 |
Final Exam 10:30 - 12:30 AM | |
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Student Responsibilities |
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As students, you are responsible for reading the assigned text before attending class. Most chapters are around 10 pages. If you have questions, write them down and bring them to class. In class, I will answer questions and may choose to review briefly some of the material. Class time will be used to extend the material in the text and explore examples. EXAMS & ASSIGNMENTS -You will not be given any midterm exams, instead you will be responsible for completing daily assignments worth 10 points each. -Points will be awarded for in-class work and quizzes (10 pts per quiz; up to 10 quizzes). -The laboratory work will be worth 20 points each -The Final Exam, worth 200 points, will only be given during the scheduled time period. The final exam format will be that of a poster session typically found at professional meetings. During part of the period the student will be at the posters to answer questions and discuss their proposed answers to the final exam questions with other students and the invited faculty. Students and faculty will also evaluate each poster based on a set of criteria that will reflect the scientific merit.
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Make-up of daily assignments will be allowed only under the following circumstances:
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Grading |
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You are responsible to see that exams and assignments are submitted on time! Grades will be assigned as shown below:
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Policies |
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Academic Integrity - It is expected that each student will do his/her own work at all times and contribute equitably in all group projects. Academic Misconduct - Dishonesty, in any form (cheating on quizzes or exams, plagiarism, copying another's assignment answers, etc.) will result in a failing grade. Disabilities: "Students with disabilities who require accommodations (academic adjustments and/or auxiliary aids or services) for this course must contact the Office for Student Disability Services, Room 445, Potter Hall. The OFSDS telephone number is (270) 745-5004 V/TDD. Please DO NOT request accommodations directly from the professor or instructor without a letter of accommodation from the Office for Student Disability Services." Withdrawals: If you wish to withdraw from the course you should do so by the dates mandated by the University. Be sure you are aware of these dates because credit for the course will not be changed after the university designated time. You also cannot drop the class or Withdraw after the designated time. Be aware that it is YOUR responsibility to drop the class. Do not assume that I will do this for you. |
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Required Response *** When you have finished reading this syllabus, you must send a signed email to your instructor and indicating whether or not you will abide by this syllabus and the prescribed policies. *** |
| © 2005 by CA Rinehart | Index • Syllabus • CourseInfo LogIn • References • t |
| This material is intended for use only by WKU students registered for Biology 350. Other uses prohibited. | |