Introduction to Recombinant Genetics- Biology 350

Syllabus
Fall 2009, 9:35 - 10:35, Tuesdays and Thursdays
3109 EBS
Instructor: Claire Rinehart
Office: 121 TCNW
Phone: 270-745-5997
Office Hours: by appointment, emailclaire.rinehart@wku.edu
Textbook:
Gene Cloning and DNA analysis, An Introduction
by T.A. Brown.
Date
Topics
Book Chapter
Sept. 1
Introduction -information resources, stats  
3
Genetic Organization 1, 16
8
What is Recombinant Gene Technology 2
10
Restriction enzymes and Ligases 3
15
Restriction enzyme maps  
17
DNA Isolation -purpose, types of clones, source of material  
22
Phage isolation 4
24
Enzyme tools needed for cloning 6, + Handout
29
Cloning vectors for E.coli & other bacteria 4, 5, 10
25
Cloning vectors for Eukaryotes 11, 14
Oct. 1
Introduction of DNA into cells 12
6

Hybridization techniques
Strategies for selecting the right clone

 
13
DNA sequencing , primer synthesis 7
15
Sequence assembly 17
20
Determining coding potential of a sequence  
22
Determining the function of an unknown gene 9
27
Studying transcription, Northerns, microarrays, RT-PCR 20
29
Proteome Analysis& 2 hybrid system  
Nov. 3
Metabolomics
Making antibodies against proteins and their use
24
5
Mutagenesis 8, 19
   
10
Knockouts and siRNA inhibition of gene expression 13, 15
12
Protein expression ...continued. 21
17
Isolation of purified recombinant proteins
Determining protein structure, MALDI
22, 23
19

Basics in Forensics 

26
24
...continued  
Dec. 1
Phylogenetics and Species identification 18
3
Gene Therapy  
8
Project  
10
Project  
Dec. 17
Final Exam 10:30 - 12:30 AM  

 

 

Student Responsibilities

As students, you are responsible for reading the assigned text before attending class. Most chapters are around 10 pages. If you have questions, write them down and bring them to class. In class, I will answer questions and may choose to review briefly some of the material. Class time will be used to extend the material in the text and explore examples.

EXAMS & ASSIGNMENTS

-You will not be given any midterm exams, instead you will be responsible for completing daily assignments worth 10 points each.

-Points will be awarded for in-class work and quizzes (10 pts per quiz; up to 10 quizzes).

-The laboratory work will be worth 20 points each

-The Final Exam, worth 200 points, will only be given during the scheduled time period. The final exam format will be that of a poster session typically found at professional meetings. During part of the period the student will be at the posters to answer questions and discuss their proposed answers to the final exam questions with other students and the invited faculty. Students and faculty will also evaluate each poster based on a set of criteria that will reflect the scientific merit.

Item

#

Points Aval.

Total

Daily Assignments

26

10

260

Quizzes:

Will vary, but no more than 10

10

100

Laboratory Ex.

12

20

240

Final Exam

1

200

200

Total



800







Make-up of daily assignments will be allowed only under the following circumstances:

  • Illness, with excuse from a Physician

  • Death of a close relative

  • Prior arrangements have been made with the instructor

 

Grading

You are responsible to see that exams and assignments are submitted on time! Grades will be assigned as shown below:


A = 90% or more of the total points.
B = 80 - 89.99%
C = 70 - 79.99%
D = 60 - 69.99%
F = Below 60%
 

Policies

Academic Integrity - It is expected that each student will do his/her own work at all times and contribute equitably in all group projects.

Academic Misconduct - Dishonesty, in any form (cheating on quizzes or exams, plagiarism, copying another's assignment answers, etc.) will result in a failing grade.

Disabilities: "Students with disabilities who require accommodations (academic adjustments and/or auxiliary aids or services) for this course must contact the Office for Student Disability Services, Room 445, Potter Hall. The OFSDS telephone number is (270) 745-5004 V/TDD. Please DO NOT request accommodations directly from the professor or instructor without a letter of accommodation from the Office for Student Disability Services." 

Withdrawals: If you wish to withdraw from the course you should do so by the dates mandated by the University. Be sure you are aware of these dates because credit for the course will not be changed after the university designated time. You also cannot drop the class or Withdraw after the designated time. Be aware that it is YOUR responsibility to drop the class. Do not assume that I will do this for you. 



Required Response

*** When you have finished reading this syllabus, you must send a signed email to your instructor and indicating whether or not you will abide by this syllabus and the prescribed policies. ***

 

© 2005 by CA Rinehart Index  •  Syllabus  •  CourseInfo LogIn  •  References  •  t
This material is intended for use only by WKU students registered for Biology 350. Other uses prohibited.