Recombinant Gene Technology
450 and 450G, 3 hrs, Spring 2007, T, R 2:00-4:55 3109 CEBS
Instructor: Dr. Claire A. Rinehart Office 121 TCNW, Lab 3112
CEBS, Phones 745-5997(o), 843-9028 (h)
Prerequisites:
Introduction to Molecular Biology (Biol. 220)
The purpose of this course is to introduce students,
both conceptually and through laboratory experiences, to the methodology used
in molecular biology. Attendance
is required.
Each Tuesday an assignment will be made that will be
due the following Tuesday. Each
assignment is worth 20 points. Assignments
will require students to outline a practical approach to solving a designated
problem (15 points). The remaining
5 points will be given for submission of a brief 1 paragraph abstract on a
technique or paper in biotechnology read during the week from one of the trade
journals (Biotechniques, Science, Nature, Genetic Engineering News, American
Biotechnology Laboratory, Bio/Technology, Promega Notes, USB Comments, BRL
Focus, or online techniques center...).
These journals are not to be taken from CEBS for any reason.
During the semester you will be required to submit
proposals for each experiment.
Proposals should contain: names of group members, date, group number, title, question
to be answered or statement of purpose, experimental design (hypothesis) with
a list of variables and their ranges (if applicable), detailed
methods with reference(s), and a list of materials needed for the experiment.
Materials will not be provided for an experiment until a proposal has
been submitted and approved by the instructor.
Each student must submit reports and a poster from
their experimental data. The report will be in standard journal style
with a title, abstract, introduction, materials and methods, results,
discussion and references. Each report will be worth 100 points
and should be ~5 pages in length. The
poster will be presented during the final exam. Each student will be required
to peer-review papers from another group. The quality of the review will be graded and will be worth
30 points. Each student is required
to keep an organized notebook so that each lab group will have duplicates
of all the data and procedures recorded throughout the semester. The laboratory notebook will be worth
70 points and each experiment should have a title, written purpose, date,
page numbers, methods, reference(s) for methods, data and the date each data
was recorded. All figures, photos
and graphs should be properly labeled with a title, date, experiment name/date
and the lanes or lines labeled or properly referenced in the data section
of the notebook. Notebooks
will be graded in class on May 4 th.
There will be 50 points assigned by the instructor
for each student's care of equipment and the cleanliness of their work area.
A comprehensive final exam worth 100 points will be
given as scheduled on Monday, May 7, from 1:00 to3:00 PM.
Graduate students will also be required to write an
original short research proposal addressing a biological problem
that could be solved using recombinant gene technology (no more than 8 pages).
The question must be approved by the instructor and should not be your
thesis question. The proposal
must include: An introduction
with a statement of the problem and the relative background (i.e., related
literature), a description of the approach to be taken with an OUTLINE
of the techniques needed, a
statement of the significance of the work, and a reference section. Final
drafts of these proposals will be due in the instructor's mail box in the
main office on April 17 at 8:00 AM.
Point Summary:
Assignments (10 @ 15 points each) 150
Abstracts (10 @ 5 points each) 50
Peer reviews (1 @30 points each)
30
Reports (1 @ 100 points each)
100
Poster (1 @ 100 points each) 100
Notebook 70
Final Exam 100
Laboratory Cleanliness 50
Undergraduate
Student Total 650
Graduate student Research Proposal 100
Graduate
Student Total 750
Grades: A
= 90-100%, B = 80-89.99%,
C = 70-79.99%, D
= 60-69.99%, F = 0 - 59.99%.
"Students with disabilities who require
accommodations (academic adjustments and/or auxiliary aids or services) for
this course must contact the Office for Student Disability Services, Room 445,
Potter Hall. The OFSDS telephone number is (270) 745-5004 V/TDD. Please DO NOT
request accommodations directly from the professor or instructor without a
letter of accommodation from the Office for Student Disability Services."
Schedule
|
Date |
Lecture Topic |
Assignment |
Experiment |
Material Due |
|
Jan. 23 |
Introduction, notebooks, experimental design, proposals, Course Info, Safety |
Assignment 1-Experimental Design |
Discovery Experiment |
Questions and Hypothesis for Discovery Experiment |
|
25 |
Introduction and Description of experiment 1. |
|
Work on proposal for experiment 1 and meet with
instructor. |
Assign. 1 |
|
30 |
Lecture topic as needed. |
Assignment 2- |
Work on proposal for experiment 1 and meet with
instructor for proposal approval. Prepare materials. |
Assign. |
|
Feb. 1 |
" |
|
Exp. 1. |
|
|
6 |
" |
Assignment 3- |
Exp. 1 |
Assign. 2 and Abstract |
|
8 |
" |
|
Exp. 1 |
|
|
13 |
" |
Assignment 4- |
Exp. 1 |
Assign. 3 and Abstract |
|
15 |
" |
|
Exp. 1 |
|
|
20 |
" |
Assignment 5- |
Exp. 1 |
Assign. 4 and Abstract |
|
22 |
" |
|
Exp. 1 |
|
|
27 |
Lecture on Paper Preparation |
Prepare Paper |
Exp. 1 |
Assign. 5 and Abstract |
|
Mar 1 |
|
|
Exp. 1 |
|
|
6 |
Introduction to Experiment 2 |
Peer Review of Paper 1 |
Exp. 2 |
|
|
8 |
Lecture topic as needed. |
|
Exp. 2 |
Corrected Paper to instructor for review. |
|
20 |
" |
Assignment 6- |
Exp. 2 |
|
|
22 |
" |
|
Exp. 1 Finished |
|
|
13, 15 |
Spring Break |
|
|
|
|
27 |
" |
|
Peer review exp. 1 report. Work on exp. 2 |
|
|
29 |
Ò |
|
Work on exp. 2 |
|
|
Apr 3 |
Ò |
|
Work on exp. 2 |
|
|
5 |
|
|
Work on exp. 2 |
|
|
10 |
Ò |
|
Work on exp. 2 |
|
|
12 |
Ò |
|
Work on exp. 2 |
|
|
17 |
" |
|
Work on exp. 2 |
|
|
19 |
" |
|
Work on exp. 2 |
|
|
24 |
" |
|
Work on exp. 2 |
|
|
26 |
" |
|
Work on exp. 2 |
|
|
May 1 |
|
|
Work on exp. 2 |
|
|
May 3 |
Final exam handed out and explained. |
|
Work on exp. 2 Notebook graded by instructor |
Notebook due |
|
May 7 |
Final Exam 1:00 Ð 3:00
|
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