MAKE SURE THAT YOU ATTACH AN ABSTRACT ON A TECHNIQUES PAPER THAT YOU HAVE READ!

1.  A scientist grew insect larvae on moistened filter paper and they would mature into adults.  One day he ran out of filter paper and used some paper towels instead.  The larvae incubated on the paper towels grew, but never matured into adults but always remained larvae. He noted that each of the paper products, the filter paper and the paper towel provided sufficient moisture to maintain the insects properly. List the variables that may be associated with these observations. Develop a hypothesis. Describe how you would test your hypothesis.
 

2. A man  lives on the 44th floor of a building.  Each morning he gets in the elevator and rides to the ground floor and goes to work.  When he comes home he rides the elevator to the 22nd floor, gets off and walks up the stairs to his apartment except on rainy days when he will ride the elevator to the 44th floor.  Design a set of hypothesis that would explain this mans behavior.

3. The LacZ gene has been used extensively to quantitatively measure the strengths of various prokaryotic and eukaryotic promoters. This is accomplished by placing the gene downstream of a promoter and then assaying the amount of beta-galactosidase activity produced within the cell as judged by the production of blue product from the XGAL substrate.

Recently, the green fluorescent protein (GFP) gene has also been found to be easy to clone and tends to express its protein anywhere the gene has a promoter. How would you design an experiment to test how well the GFP gene would serve as an indicator of promoter strength?