SYLLABUS FALL 2000
GENERAL MICROBIOLOGY LABORATORY BIOLOGY- 208
Thompson Complex, North Wing, Room 200

CLIENTELE:  This course is listed under General Education Requirements Category D. It is intended for scientifically and/or technologically oriented students not majoring in biology and should be taken concurrently with Biology 207.

PREREQUISITES: No prerequisites are required for this course but introductory knowledge of biology and chemistry would be beneficial.

OBJECTIVES: To introduce and provide experience with some fundamental laboratory techniques necessary for the study of microbes.

LAB MANUAL: Wilson, Marion E., Helen Eckel Mizer, and Josephine A.Morello, 1994, Laboratory Manual and Workbook in Microbiology (Applications to Patient Care.) Fifth Edition, Wm. C. Brown Publishers, Dubuque, IA. All students are required to purchase a new laboratory manual. Use of previously used lab manuals is not permitted.

ATTENDANCE: Every laboratory period must be attended punctually and entirely (See section on GRADING).

EXAMS: Three exams will be given. Exam I (50 points)will be given during the first hour of period 5 and will cover material from labs 1-4. Exam II (50 points) will be given the first hour of or prior to period 9 and will cover material from labs 5-7. Exam III (100 points) will be given during Period 13 and cover material from labs 8-12. Exams II and III

Review. http://bioweb.wku.edu/faculty/Clauson

GRADING: Each student's Term Letter Grade (TLG) will be determined by the scores earned on the three exams (200 total points) plus credit for each of 12 laboratory periods in which each student is required to complete and record exercise data. The instructor reserves the right to randomly request your laboratory data pages to assure their completion and evaluate performance. Five (5) points will be earned after completing each laboratory period and recording the data from that period in your laboratory manual.

These points will be summed to obtain a Cumulative Score. The maximum number of points awarded will be 200 (test scores) + 60 (12 completed laboratories) = 260

Basis for Assigning each TLG

234 points A
208 points B
182 points C
156 points D
Below 156 F

SCHEDULE

PERIOD WEEK OF   Exercise
1 08-22-00 1. The Microscope
    2. Handling and Examining Cultures 
2 08-29-00  3. The Hanging-Drop Preparation
    4. Simple Stains
3. 09-05-00  5. Gram stain
    7.1 Special stains, Endospore stain
    9. Pure culture technique
4. 09-12-00 11.  Culturing Microorganisms from the Environment
    12. Moist and Dry Heat
    13. The Autoclave
    14. Disinfectants
5. 09-19-00

 EXAM I (labs 1-4)

    15. Antibiotics
   

16. Primary Media for Isolation of Microorganisms

6.

09-26-00

17. Some Metabolic Activities of Bacteria

   

18. Activities of Bacterial Enzymes

7.

10-10-00 

19. Streptococci, Pneumococci and Enterococci
   

20. Staphylococci

 

10-16-00

 last day to drop the lab with a (W)
8. 10-17-00  21. Klebsiella and Haemophilus
    22. Corynebacteria and Bordetella
    23. Clinical Specimens from the Respiratory Tract
9.   10-24-00  EXAM II (labs 5-7)
    26. Urine Culture Techniques
    27. Neisseria and Spirochetes
10.

10-31-00

28. Anaerobic Bacteria
    29. Mycobacteria
    31. Yeast and other Fungi
11. 11-14-00  24. Enterobacteriaceae (Enteric Bacilli)
    24.5 Rapid Methods of Bacterial Identification
    33. Serologic Identification of Microorganism
    34. Serologic Identification of Patients Antibodies
12. 11-28-00  Results, Laboratory 11 - REVIEW WEEK
13. 12-05-00  FINAL EXAM (labs 8-12)

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