- Jacobshagen Lab

Research Projects

  • Analyzing the frequency of circadian gene expression in Chlamydomonas
  • Studies on the transcription of rhythmically expressed genes
  • Promoter analysis to determine circadian regulatory sequences
  • Isolating mutants in the circadian output pathway
  • Establishing luciferase as a reporter gene for Chlamydomonas
  • Gene expression analysis in circadian period mutants
Student Researchers
  • Xavier Basseda: Isolation of the cell wall-degrading enzyme autolysin and transformation of Chlamydomonas with a ß-tubulin promoter/arylsulfatase reporter gene fusion.
  • Scott Myers: Identifying the minimal lethal dose of the antibiotic zeocin for Chlamydomonas and isolating resistant strains through transformation with a zeocin resistance gene.
  • Matt Boling: Propagation of plasmids for use as probes in northern and southern analyses.
  • Frank Valenzuela: Cloning of plasmids with deletions in the CABII-1 promoter.
Publications:
  • S. Jacobshagen, K.L. Kindle and C.H. Johnson (1996): Transcription of cabII is regulated by the biological clock in Chlamydomonas reinhardtii. Plant Mol. Biol. 31, 1173-1184
  • S. Jacobshagen and C.H. Johnson (1994). Circadian rhythm of gene expression in Chlamydomonas reinhardtii: circadian cycling of mRNA abundances of cab II, and possibly of ß-tubulin and cytochrome c. Eur. J. Cell Biol. 64, 142-152.
  • C. Schnarrenberger, B. Pelzer-Reith, H. Yatsuki, S. Freund, S. Jacobshagen and K. Hori (1994): Expression and sequence of the only detectable aldolase in Chlamydomonas reinhardtii. Arch. Biochem. Biophys. 313, 173-178.
  • C. Schnarrenberger, W. Gross, B. Pelzer-Reith, S. Wiegand and S. Jacobshagen (1992): The evolution of isoenzymes of sugar phosphate metabolism in algae. In: Phylogenetic changes in peroxisomes of algae - Phylogeny of plant peroxisomes. H. Stabenau (ed.), University of Oldenburg Press, Oldenburg, Germany, pp 310-329.
  • C. Schnarrenberger, S. Jacobshagen, B. Müller and I. Krüger (1990): Evolution of isozymes of sugar phosphate metabolism in green algae. In: Isozymes: Structure, Function, and Use in Biology and Medicine. M.C. Rattazzi, J.G. Scandalios and G.S. Whitt (eds), Alan R. Liss Inc., New York, pp 743-764.
  • S. Jacobshagen and C. Schnarrenberger (1990): Two class I aldolases in Klebsormidium flaccidum (Charophyceae) - an evolutionary link from Chlorophytes to higher plants. J. Phycol. 26, 312-317.
  • S. Jacobshagen and C. Schnarrenberger (1988): Two class I aldolases in the green alga Chara foetida (Charophyceae). Plant Physiol. 87, 78-82.
  • S. Jacobshagen, D. Altmüller, F. Grolig and G. Wagner (1986): Calcium pools, calmodulin and light-regulated chloroplast movements in Mougeotia and Mesotaenium. In: Molecular and cellular aspects of calcium in plant development. A. J. Trewavas (ed.), Plenum press, New York-London- Washington D.C.-Boston, pp 201-209.
  • S. Jacobshagen, F. Grolig and G. Wagner (1986): Quantification of Mesotaenium calmodulin by improved cyclic nucleotide phosphodiesterase test. In: Molecular and cellular aspects of calcium in plant development. A. J. Trewavas (ed.), Plenum press, New York-London-Washington D.C.-Boston, pp 381-382.
Education
  • Ph.D. in biology, Free University of Berlin, Germany 1988. Thesis title: "Evidence for cytosol- and chloroplast-specific class I-aldolases in green algae and their comparison".
  • M.S. in biology; Justus-Liebig-University of Giessen, Germany 1985. Thesis title: "Isolation and purification of calmodulin from Mesotaenium caldariorum by affinity chromatography".