Research Projects:

Analyzing the frequency of circadian gene expression in Chlamydomonas
Studies on the transcription of rhythmically expressed genes
Promoter analysis to determine circadian regulatory sequences
Isolating mutants in the circadian output pathway
Establishing luciferase as a reporter gene for Chlamydomonas
Gene expression analysis in circadian period mutants

Teaching:

Plant Physiology [BIOL 400]
Biochemistry I [BIOL/CHEM 446]
Biochemistry II [BIOL/CHEM 467]
Biochemistry Laboratory [BIOL/CHEM 447]
Advanced Biochemistry [BIOL/CHEM 562]
Nucleic Acids [BIOL 588]
Introduction to Molecular and Cell Biology [BIOL 220]
Biological Concepts [BIOL120]

Education: Ph.D. in biology, Free University of Berlin, Germany 1988.

Thesis title: "Evidence for cytosol- and chloroplast-specific class I-aldolases in green algae and their comparison".

M.S. in biology; Justus-Liebig-University of Giessen, Germany 1985.

Thesis title: "Isolation and purification of calmodulin from Mesotaenium caldariorum by affinity chromatography".

 

Student Researchers:

Xavier Basseda: Isolation of the cell wall-degrading enzyme autolysin and transformation of Chlamydomonas with a ß-tubulin promoter/arylsulfatase reporter gene fusion.

Scott Myers: Identifying the minimal lethal dose of the antibiotic zeocin for Chlamydomonas and isolating resistant strains through transformation with a zeocin resistance gene.

Matt Boling: Propagation of plasmids for use as probes in northern and southern analyses.

Frank Valenzuela: Cloning of plasmids with deletions in the CABII-1 promoter.

 

Publications:

S. Jacobshagen, K.L. Kindle and C.H. Johnson (1996): Transcription of cabII is regulated by the biological clock in Chlamydomonas reinhardtii. Plant Mol. Biol. 31, 1173-1184
S. Jacobshagen and C.H. Johnson (1994). Circadian rhythm of gene expression in Chlamydomonas reinhardtii: circadian cycling of mRNA abundances of cab II, and possibly of ß-tubulin and cytochrome c. Eur. J. Cell Biol. 64, 142-152.
C. Schnarrenberger, B. Pelzer-Reith, H. Yatsuki, S. Freund, S. Jacobshagen and K. Hori (1994): Expression and sequence of the only detectable aldolase in Chlamydomonas reinhardtii. Arch. Biochem. Biophys. 313, 173-178.
C. Schnarrenberger, W. Gross, B. Pelzer-Reith, S. Wiegand and S. Jacobshagen (1992): The evolution of isoenzymes of sugar phosphate metabolism in algae. In: Phylogenetic changes in peroxisomes of algae - Phylogeny of plant peroxisomes. H. Stabenau (ed.), University of Oldenburg Press, Oldenburg, Germany, pp 310-329.
C. Schnarrenberger, S. Jacobshagen, B. Müller and I. Krüger (1990): Evolution of isozymes of sugar phosphate metabolism in green algae. In: Isozymes: Structure, Function, and Use in Biology and Medicine. M.C. Rattazzi, J.G. Scandalios and G.S. Whitt (eds), Alan R. Liss Inc., New York, pp 743-764.
S. Jacobshagen and C. Schnarrenberger (1990): Two class I aldolases in Klebsormidium flaccidum (Charophyceae) - an evolutionary link from Chlorophytes to higher plants. J. Phycol. 26, 312-317.
S. Jacobshagen and C. Schnarrenberger (1988): Two class I aldolases in the green alga Chara foetida (Charophyceae). Plant Physiol. 87, 78-82.
S. Jacobshagen, D. Altmüller, F. Grolig and G. Wagner (1986): Calcium pools, calmodulin and light-regulated chloroplast movements in Mougeotia and Mesotaenium. In: Molecular and cellular aspects of calcium in plant development. A. J. Trewavas (ed.), Plenum press, New York-London- Washington D.C.-Boston, pp 201-209.
S. Jacobshagen, F. Grolig and G. Wagner (1986): Quantification of Mesotaenium calmodulin by improved cyclic nucleotide phosphodiesterase test. In: Molecular and cellular aspects of calcium in plant development. A. J. Trewavas (ed.), Plenum press, New York-London-Washington D.C.-Boston, pp 381-382.